Validation of a quantitative PCR assay for the detection and quantification of the nematode Litylenchus crenatae in American beech leaf tissue
The development and testing of a probe-based qPCR detection assay that integrates previously reported LC-specific primers with a newly designed probe, enabling sensitive and reproducible quantification of nematodes in plant and non-plant matrices is reported, supporting the utility of the assay for monitoring and surve...