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Author

Elise D. Ficaretta

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Open access Sep 2026

Development of m6A Readers for Enhanced Recognition In Vitro via Genetic Code Expansion

The YTH domain was recently demonstrated as a promising tool for recognizing and regulating m6A-modified RNAs. However, the deficiency in binding affinity (micromolar range) and selectivity (off-target binding) for m6A of the wild-type YTH domain limit its sensitivity and accuracy for m6A recognition. Engineering strat...

Michael S. Addo, Elise D. Ficaretta, Yi-Bo Chang et al. · 0 citations
Open access Sep 2026

Repurposing engineered aminoacyl-tRNA synthetases for proximity-dependent protein labeling through the release of aminoacyl-AMP intermediate

Over the past two decades, engineering efforts have yielded aminoacyl-tRNA synthetases (aaRSs) capable of charging diverse noncanonical amino acids (ncAAs). Here, we report an unexpected and exploitable activity of these engineered aaRSs: In the absence of their cognate tRNA, aaRSs can release the ncAA-AMP intermediate...

T. Yared, Elise D. Ficaretta, Lena A. Voss et al. · 0 citations

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