Paired CYP1A2/3A4-HepG2 phenotyping supports machine-learning classification of literature-curated hepatotoxicity categories.
Limited CYP1A2/3A4 activity in wild-type HepG2 cells can obscure hepatotoxic responses that require metabolic activation. We established matched CYP1A2/3A4-co-expressing and empty-vector (EV) HepG2 cells and profiled 81 compounds with six high-content imaging readouts and three extracellular injury biomarkers. Absolute...