Rapid subtype-specific detection of respiratory syncytial virus A and B using an RNase HII-dependent RT-LAMP lateral flow assay
Introduction Rapid and simultaneous detection of respiratory syncytial virus A (RSV A) and RSV B with subtype discrimination is important for point-of-care (POC) diagnostics and epidemiological surveillance. Methods We developed the RSV A/B rh-RT-LAMP-NALF assay, a duplex reverse transcription loop-mediated isothermal...