How many gamma delta T cells are there? Calling strategy, quality control and receptor-locus specificity in 3-prime single-nucleus RNA sequencing of human small intestine.
Public single-nucleus transcriptomes are increasingly reused to count gamma delta T cells in the human small intestine, but how far that count depends on analytical choices has not been quantified. Using a public 3-prime single-nucleus dataset of 33,600 nuclei from six small-intestinal biopsies, we benchmarked three ca...