Ancestral Sequence Reconstruction Synergized with Deep Learning Significantly Enhances the Thermophilicity and Thermostability of the Highly Active Alkaline Protease AprE
Although alkaline proteases have broad industrial applications, their practical use is hindered by insufficient thermostability and limited strategies to elevate optimal catalytic temperature (Topt) while retaining catalytic activity. To enhance Topt and thermostability of Bacillus clausii AprE without sacrificing ca...