Author

F. Zhu

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Sep 2026

A CRISPR/Cas14a-based platform for sensitive and specific single-nucleotide variant detection via short single-strand DNA amplification and split target design.

Single-nucleotide variant (SNV) detection, including tumor-associated mutation detection and pharmacogenetic genotyping, holds significant clinical value. Among current methods, CRISPR/Cas14a-based approaches offer advantages in specificity, sensitivity, and efficiency. However, the inherent single-stranded DNA (ssDNA)...

Yi-Bo Hu, Yang-Wei Liao, Xiao-Xiang Wang et al. · Biosensors & bioelectronics · 0 citations

Aug 2026

Double-end blocker and split input mediated CRISPR-Cas12a system for modular single-base mutation detection with low abundance.

A crRNA-universal, sensitive and specific CRISPR-Cas12a detection platform, termed DESIC (double-end blocker and split-input mediated CRISPR-Cas12a system), for single-base mutation detection, targeting four prevalent pancreatic cancer KRAS mutations and optimized the system to achieve optimal discrimination.

Shi-Zhen Li, Yang-Wei Liao, Xiao-Xiang Wang et al. · Biosensors & bioelectronics · 0 citations