Establishment and Application of a Triplex Real-Time Fluorescent Quantitative RT-PCR Assay for Simultaneous Detection of H5, H7 Subtype AIV and Virulent NDV.
To rapidly monitor clinical infections induced by highly pathogenic avian influenza (HPAI) of H5/H7 subtypes and virulent Newcastle disease virus (NDV), mitigate economic losses in intensive poultry industry and reduce zoonotic spillover risks to human population, a one-tube triplex TaqMan real-time RT-qPCR was develop...