Reprogramming Enfumafungin Biosynthesis for High-Titer Production in the Endophytic Fungus Hormonema carpetanum ATCC 74360
Enfumafungin is the essential precursor for the FDA-approved antifungal agent Ibrexafungerp, yet its production is hindered by low yields, restrictive solid-state fermentation, and the genetic intractability of its producer strain, Hormonema carpetanum ATCC 74360. Here, we developed a CRISPR/Cas9-mediated platform th...