Mandatory use of mock communities highlighted by the descriptive comparison of Epi2Me 16S and EMU bioinformatic workflows for full-length 16S rRNA Nanopore sequencing
The results highlight that Epi2Me integrated workflows are not the best option for specie-level taxonomic assignation and underscores the importance of routine inclusion of positive controls for microbiota studies, in the form of mock communities, as a critical safeguard for accurate data interpretation.