A newly established reverse genetic system for a circular RNA virus reveals new requirements for infection and its biocontrol potential
This work constructed the first infectious cDNA clone of an ambivirus, Fusarium graminearum ambivirus 1 (FgAV1), using a head-to-tail dimer placed downstream of a fungal promoter, demonstrating that FgAV1 infection triggers a fungal RNAi response, extending the antiviral role of host sRNAs to circular RNA viruses.