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It is shown that MclX regulates mother cell lysis by inhibiting SpoIIID, a sporulation-specific transcription factor that represses cwlC, which encodes a key hydrolase required for lysis.
This work utilized an AlphaFold3-based structure-prediction approach to identify candidate genes and developed a Bt chassis strain that can be universally used to express new pesticidal proteins, providing a versatile framework for next-generation biocontrol and sustainable pest-management innovation.
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