From Gibson Assembly to Cell-Free Expression: a guide to Recombinant Plasmid and Toscana Virus N Protein Production.
Toscana virus (TOSV) is a mosquito-borne phlebovirus endemic to the Mediterranean region and an important cause of arboviral infections in humans, particularly during the summer months. Although TOSV infections are often asymptomatic or mild, the virus can cause neuroinvasive disease, including meningitis and encephali...