Author

G. Kim

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2026

Construction of a Tl-CRISPRi Genetic Circuit in Bacteria for Translation-Level Gene Knockdown.

This chapter describes how to design spacer sequences and install them into the guide RNA expression plasmid and describes how to mutate the handle of gRNA to achieve tunable knockdown of a target gene.

G. Kim, H. Kim, Sang Woo Seo · Methods in molecular biology · 0 citations