Enhancing TGF-β1 production in CHO cells via MS-275 treatment, Bax/Bak knockout, and PACEsol overexpression
Efficient production of recombinant human transforming growth factor-β1 (rhTGF-β1) in Chinese hamster ovary (CHO) cells is limited by low yields and inefficient precursor processing. Here, we developed an integrated strategy combining chemical and genetic engineering. Screening of additives revealed that the histon...