Author

G. van Mierlo

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Open access Aug 2026

Identification of methylation-sensitive human transcription factors using meSMiLE-seq

Transcription factors (TFs) are key players in eukaryotic gene regulation, but the DNA binding specificity of many TFs remains unknown. Here, we assay 284 mostly uncharacterized putative human TFs using selective microfluidics-based ligand enrichment followed by sequencing (SMiLE-seq), revealing 74 new DNA binding moti...

Antoni J. Gralak, Kateřina Faltejsková, Ally W. H. Yang et al. · Nature Communications · 2 citations

Open access Aug 2026

TSPAN3 defines a distinct intracellular trafficking route to secretory multivesicular bodies

Extracellular vesicles (EVs) comprise molecularly diverse populations generated through multiple membrane-trafficking pathways, yet the intracellular basis of this heterogeneity remains poorly understood. Here, we identify the EV-associated tetraspanin TSPAN3 as a marker of a secretory multivesicular body (MVB) populat...

Jelle van den Bor, Misko Bobeldijk, C. A. Zala et al. · bioRxiv · 0 citations