Gaurav Pandey

Designing an optimized strategy for extracellular expression of recombinant human TNF-α in Escherichia coli

Extracellular protein expression in Escherichia coli is an elegant solution that addresses the complex issue of protein misfolding while simultaneously simplifying downstream processing steps. Human TNF-α was chosen as the target protein for export since it is a therapeutically important cytokine. Different genomic kno...