Gerald Striedner

Metabolic Engineering and Bioprocess Optimization Strategies Promote l -Azidohomoalanine Biosynthesis in Fed-Batch Bioreactor Culture

The noncanonical amino acid l-azidohomoalanine (Aha) can replace methionine in ribosomal translation under tightly controlled conditions. Aha has been commonly employed to functionalize proteins by the bioorthogonal conjugation with alkynes, the so-called “click chemistry”. However, its application for scalable recom...