mBACS enhances pseudouridine profiling through efficient and robust chemical conversion
mBACS revealed TRUB1 and PUS10 as the exclusive redundant writers of the conserved tRNA Ψ55 modification and uncovered crosstalk between Ψ55 and other tRNA modifications, and supported robust Ψ profiling from as little as 10 ng of total RNA, establishing it as a sensitive, quantitative, and low-input second-generation...