Double-end blocker and split input mediated CRISPR-Cas12a system for modular single-base mutation detection with low abundance.
A crRNA-universal, sensitive and specific CRISPR-Cas12a detection platform, termed DESIC (double-end blocker and split-input mediated CRISPR-Cas12a system), for single-base mutation detection, targeting four prevalent pancreatic cancer KRAS mutations and optimized the system to achieve optimal discrimination.