Guo-Cheng Du

Combinatorial optimization of ribosomal binding site, promoter and genomic locus for high-yield production of a thermostable transglutaminase in Streptomyces mobaraensis.

Streptomyces mobaraensis transglutaminase (smTG) is an important food-processing biocatalyst, yet its industrial potential is limited by low yield and thermal instability. Here, we report the multi-level engineering of S. mobaraensis smY2022 for high-level production of a thermostable variant, TGm2. Ab initio design of...