Enzymatic Characterization and Application Study of Fructosyl Peptide Oxidase with Improved Thermal Stability and Activity
Abstract Fructosyl peptide oxidase (FPOX) is clinically valuable for glycated hemoglobin (HbA1c) quantification, but current variants suffer from low activity and thermal stability, limiting their applications. A novel consensus sequence (FPOX-Con) was designed by analyzing the structural and sequence data of existing...