Autophagy regulates the function of myeloid-derived suppressor cells in tumor-induced immunosuppression
Tumor-infiltrating MDSCs were isolated from syngeneic C57BL/6 mouse models bearing B16-F10 melanoma or EO771 breast carcinoma tumors. Immunofluorescence staining of LC3B and western blotting of p62/SQSTM1 were used to assess autophagic activity. T-cell suppression by MDSCs was evaluated using co-culture assays, includi...