Methylene and difluoromethylene uridine bisphosphonate carbagalactose were designed and synthesized as stable uridine diphosphate-galactose (UDP-Gal) mimics for probing α-1,4-galactosyltransferase (A4GALT). In these analogues, the labile glycosidic and pyrophosphate linkages of UDP-Gal are replaced by chemically and en...
Jian-Yun Guo, Nicky de Koster, Bob van Puffelen et al.· Organic Letters· 0 citations
These findings define functional and structural diversity within GH172 and establish chemical probes for profiling α-ᴅ-arabinofuranosidase activity in complex biological samples.
Jennifer Ross, Moescha J. Hoopman, Florian Küllmer et al.· bioRxiv· 0 citations
The synthesis of cyclophellitol-inspired ABPs based on mannobiose, mannotriose, and glucomannose are described and their use in reporting mannanase activities in secretomes from saprophytic bacteria and fungi grown on mannan-containing biomass polysaccharides is described.
Massimo Tedeschi, Vincent A. J. Lit, Nicholas G. S. McGregor et al.· Chemical Science· 1 citation
The molecular mechanism of human A4GALT is revealed at atomic detail using QM/MM simulations, revealing a conformational rearrangement involving a 310-helix that stabilizes the donor substrate and promotes a front-face SNi-like catalytic mechanism, in which a short-lived oxocarbenium-ion intermediate forms.
Nicky de Koster, Òscar Vidal-Gironès, Rowan de Graaf et al.· Angewandte Chemie· 0 citations
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