Cellobiose dehydrogenase (CDH) is a fungal extracellular flavocytochrome crucial for biomass degradation and promising for bioelectrocatalytic applications. However, its practical application remains limited by suboptimal catalytic efficiency and inefficient electron transfer. Here, an integrated engineering strategy w...
Xin-Yu Cui, Hai-Yan Song, Yuan-Ming Wang et al.· ChemElectroChem· 0 citations
Cellobiose dehydrogenase (CDH) is a typical biomass catalyst with significant potential for applications such as lignocellulose degradation, bioremediation, and bioelectrocatalysis. Its two-domain flavoheme structure enables the construction of direct electron transfer (DET)-based bioelectrochemical devices. However,...
Xin-Yu Cui, Hai-Yan Song, Yuan-Ming Wang et al.· ACS Catalysis· 0 citations
Efficient use of reducing equivalents is critical for productive turnover in cytochrome P450 redox enzymes, yet improving activity without compromising substrate affinity remains challenging. Here, we engineered the second-sphere region of PbdA, a cytochrome P450 from Rhodococcus jostii RHA1 that catalyzes veratrate ox...
Hai-Yan Song, Ze-Yang Li, An-Dong Li et al.· Bioresources and Bioprocessi...· 0 citations
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