Hai-Yang Hu

Structural and catalytic characterization of the 2,5-dihydroxypyridine ring-cleaving dioxygenase Hpo from Pseudomonas putida S16

ABSTRACT 2,5-Dihydroxypyridine (2,5-DHP) is a potentially carcinogenic intermediate in pyridine catabolism. Hpo, a non-heme Fe(II)-dependent dioxygenase from Pseudomonas putida S16, catalyzes ring cleavage of 2,5-DHP, yet its structure has remained uncharacterized. We determined the crystal structure of Hpo at 2.56 Å r...