Development of PEG-Mediated Genetic Transformation for Pine-Inhabiting Fungus Pestalotiopsis disseminata
Pestalotiopsis disseminata widely inhabits pines, but the lack of an efficient genetic transformation system has limited its functional genomic studies. Here, we optimized protoplast preparation and established a PEG-mediated transformation and GFP-labeling platform for strain ZXD579. The selected conditions used mycel...