Open access
Oct 2026
SaCas9-Mediated CrtYB Disruption Coupled with Heterologous α-Bisabolol Production in Rhodotorula mucilaginosa JY1105
Rhodotorula mucilaginosa is a promising host for terpene biosynthesis, but its rational engineering remains constrained by limited genome-editing tools. Here, we developed a Staphylococcus aureus Cas9 (SaCas9) workflow in R. mucilaginosa JY1105 for CrtYB disruption and demonstrated heterologous α-bisabolol production....
Wen-Yi Sun, Hao-Yang Liu, Ya-Qi Dong et al.
· Microorganisms · 0 citations