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Author

Heinz Koeppl

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Open access Oct 2026

Releasing Protein Cargo from Synthetic Cells by Coupling Cell-Free Expression to Osmotic Actuation

Macromolecular release from synthetic cells often relies on membrane pores or channels. We explored whether internally generated osmotic stress could increase membrane permeability and promote release. Using double-emulsion microfluidics, we encapsulated an Escherichia coli cell-free expression system in Pluronic L121...

Hong-Fei Liu, Jérémie J. M. Marlhens, Simon Schubotz et al. · 0 citations
Open access Sep 2026

A Yeast Logic Toolkit for Fast and Efficient Assembly of Genetic Circuits

Synthetic biologists aim for standardization and simplicity in order to ensure reproducibility and reliability. Toolkits offer both, making it easier to share DNA sequences between labs while minimizing context dependent effects from sequences surrounding the construct of interest. The Yeast Toolkit (YTK) for the model...

Maik Molderings, Erik Kubaczka, Heinz Koeppl · 0 citations

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