Genome Characterization of a Novel Bisegmented Positive-Sense RNA Virus Infecting the Bean Flower Thrips (Megalurothrips usitatus) Reveals an Active Antiviral RNAi Response
By combining next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE) PCR, we sequenced from adult bean flower thrips (Megalurothrips usitatus) the near-complete genome of a previously undescribed virus (related to Anopheline-associated C vi...