Open access
Sep 2026
Exploring the Substrate Selectivity of the Condensation Domain VibH through Engineered Adenylation Domains, Non-Native Amide Synthesis, and Substrate Recognition Analysis.
A systematic functional analysis of VibH, a stand-alone C domain VibH from the vibriobactin biosynthetic pathway, reveals distance-dependent steric tolerance and mechanistic insights into C-domain specificity and establishes a framework for rational NRPS reprogramming.
F. Ishikawa, Hiroyuki Izuno, Shinya Nakamura et al.
· ACS Chemical Biology · 0 citations