3'-Sialyl-3-fucosyllactose (3'S3FL) is a representative fucose-containing sialylated human milk oligosaccharide with distinctive biological functions, but its in vitro synthesis is limited by the poor tolerance of α2,3-sialyltransferases toward fucosylated acceptors. Here, we report an engineered α2,3-sialyltransferase...
Yi-Fan Cao, Rui Chen, Tong-Yan Yin et al.· Journal of Agricultural and...· 0 citations
Although H2O2-mediated degradation efficiently yields bioactive oligosaccharides, the underlying mechanisms and structure–activity relationships (SARs) of agar polysaccharides remain elusive. Here, the H2O2 degradation profiles of agar (FA) and agarose (TA) were systematically compared using HRMS/MS and 2D NMR. FA pr...
Chao-Dong Yi, Jiang-Bin Shi, Bo-Lun Li et al.· Journal of Agricultural and...· 0 citations
Chitosanases are attractive biocatalysts for producing bioactive chito-oligosaccharides (COS), but their applications are limited by insufficient catalytic efficiency, poor stability, and challenging recovery. Although many chitosanases can be activated by metal ions, the direct addition of free ions into reaction syst...