Hongxia Li

Synchronous dual-Cas12a activation via dimeric palindromic hairpin-programmed cascade amplification for ultrasensitive molecular diagnostics.

Integrating isothermal nucleic acid amplification with CRISPR/Cas12a trans-cleavage has emerged as a powerful strategy for ultrasensitive molecular diagnostics. However, most systems depend on multiple probes, separated amplification modules, or complex probe networks, increasing design complexity, optimization burden,...