The trans-cleavage activity of CRISPR/Cas systems has catalyzed significant progress in molecular diagnostics. Compared with traditional methods such as polymerase chain reaction (PCR) and its derivatives, CRISPR/Cas diagnostics are often credited with high specificity, portability, and visual readout. Among various CR...
Rong Gao, Hong-Li Jin, Tian-Yi Zhang et al.· Biotechnology Advances· 0 citations
ABSTRACT Rabies virus (RABV), a lethal neurotropic pathogen, relies on interaction with the host to sustain neuronal infection; however, how it balances replication with neuronal survival remains unclear. Here, we revealed Snapin, a neuronal trafficking regulator, to be a critical host factor co-opted by RABV to enhanc...
Yu-Jie Bai, Yong-Sai Liu, Xiao-Yu Wu et al.· Journal of Virology· 0 citations
A visual nucleic acid detection assay for SBV that combines reverse transcription recombinase‑aided amplification (RT‑RAA) with the CRISPR/Cas12a system, targeting conserved regions of the SBV S gene holds promise for further clinical validation, although its diagnostic utility in authentic clinical specimens remains t...
