Huan-Jie Zhu

Possible origin and suppression of apparent +56 Da impurities during peptide synthesis.

Apparent +56 Da signals present a significant challenge in therapeutic peptide characterization. Using a 36-residue carbohydrate-binding module (CBM) as a representative model, we systematically investigated the chemical origins and analytical behavior of these species. The major chromatographically separable +56 Da im...