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Huihui Sun

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Jul 2026

Bidirectional Loop Engineering Uncovers a Structural Determinant for Product Specificity in GH46 Family Chitosanases.

A bidirectional loop-engineering strategy is employed to decipher the function of a conserved loop (XDXXP) in GH46 chitosanases and reveals that the loop modulates the geometry, electrostatics, and hydrophilicity of the substrate-binding cleft, thereby influencing substrate recognition and product specificity.

Mao-Yue Li, Minjuan Gui, Yingying Wei et al. · 0 citations