I. Tarassov

Modified crRNA variants for precise targeting of point mutations in human mitochondrial DNA by CRISPR-Cas12a

The CRISPR-Cas12a system, primarily used for nuclear genome editing, operates through RNA-guided cleavage of target DNA. Given the high copy number of mitochondrial DNA (mtDNA) in cells and the pathogenic potential of mtDNA mutations, there is substantial interest in developing methods to selectively eliminate mutant m...