Open access
Sep 2025
Structural basis for target discrimination and activation by Cas13d
The mechanisms of nuclease activation are explored by solving seven ternary cryo-electron mi-croscopy structures of wild-type Cas13d in complex with matched and mismatched targets and an active site loop in the HEPN domains that regulates substrate accessibility is identified.
Chia-Wei Chou, Selma Sinan, Hung-Che Kuo et al.
· bioRxiv · 0 citations