Review
Open access
Jul 2026
Mapping the dynamic plant interactome: from in vitro assays to in vivo quantitative approaches
A practical, integrative three-tier framework is proposed, combining proximity labeling for broad in vivo discovery, split-luciferase complementation for sensitive binary validation, and fluorescence lifetime imaging microscopy for quantitative confirmation, that systematically funnels candidate interactions from initi...
Muhammad Ans Hussain, A. H. Hafeez, Iqra Noor et al.
· Plant Methods · 0 citations