Aug 2026
In vitro CRISPR/Cas9–RNP cleavage of CcYUC1 in Coffea canephora
An in vitro CRISPR/Cas9–RNP system efficiently cleaves CcYUC1 in Coffea canephora, establishing a foundation for DNA-free genome editing in coffee and providing a basis for future functional studies of auxin biosynthesis during somatic embryogenesis.
Miguel A. Uc-Chuc, V. Aguilar-Hernández, Irma A. Jiménez-Ramírez et al.
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