Author

J. Hobson-Peters

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Open access Aug 2026

Expanding Reverse Genetics of Positive-Strand RNA Viruses: Optimised Rescue Platforms and Construction of a Novel Fluorescent Reporter Nidovirus

It is demonstrated that a potent constitutive promoter system and a hammerhead ribozyme enhance the efficiency of positive-sense RNA virus rescue using CPER, and may support applications requiring efficient recovery of low fitness mutants and diverse mutational libraries and barcoded virus populations.

James R. Potter, Helen Mostafavi, A. Amarilla et al. · bioRxiv · 0 citations

Open access Aug 2026

Sequence Homology and Tissue Tropism Determine Superinfection Exclusion of Zika virus in Aedes aegypti Mediated by an insect-specific Binjari-Zika Virus Chimera

It is demonstrated that incorporation of Zika virus prM and E proteins into the ISF backbone enhances infection of the mosquito midgut following oral exposure, which enables direct inhibition of ZIKV infection after a subsequent infectious blood meal, and defines a replication-dependent, tissue-specific mechanism of IS...

Wessel Willemsen, Alyssa J. Peterson, Marleen Henkens et al. · bioRxiv · 0 citations