Open access
Jul 2026
Reprogramming a Protein Ligase for Genetic Code Expansion
It is demonstrated that chEpmA serves as a versatile dual-specificity platform: it efficiently charges tRNAs with the non-canonical backbone (R)-β-lysine, and a single substitution unlocks the scaffold for α-substrates, thereby enabling a broad spectrum of post-translational modifications previously inaccessible to gen...
Giovanni Gallo, Alina Sieber, Michael Hellwig et al.
· bioRxiv · 0 citations