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Author

J. Mittal

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Open access Aug 2026

Enhancing Enzyme Activity Via Noncovalent Association with DNA-Functionalized Nanoparticles

Enhancing enzyme performance without protein engineering remains a central challenge in biocatalysis. Here, we report the unusual discovery that nanoparticles densely functionalized with DNA, commonly known as spherical nucleic acids (SNAs), can bind enzymes without chemical conjugation, affinity tags, or engineered re...

Seungheon Lee, Atri Bhattacharya, Regan Kuttler et al. · 0 citations
Open access Sep 2026

Molecular Details of RNA Interactions With FUS Condensates Altering Assembly, Dynamics, and Aggregation.

It is demonstrated how RNA modulates the phase separation of the low-complexity (LC) and arginine-glycine-glycine-glycine motif (RGG1) domains of FUS-low RNA concentrations enhance protein phase separation and excess RNA disrupts it.

Tong-Yin Zheng, Kandarp A. Sojitra, Samara Cummings et al. · 0 citations
Open access Sep 2026

Molecular origins of pH gradients in charge-regulated biomolecular condensates.

The buffered Charge-Regulation Monte Carlo (b-CR-MC) framework is introduced, which couples grand-canonical exchange of ions and buffer species with explicit charge regulation of titratable residues and enables quantitative prediction of how mutations and post-translational modifications reprogram condensate microenvir...

Shuo-Lin Weng, Shiv Rekhi, Young C. Kim et al. · 0 citations
Open access Sep 2026

Scaffold Affinity Tunes Biomolecular Condensate Function

It is found that affinity governs the phase boundary, resistance to chemical perturbation, and molecular mobility of condensates in vitro and in human cells and is shown to be a quantitative determinant of condensate phase behavior, internal dynamics and biochemical output.

Andres Reyna, Madyson O. Briggs, Alexander F. Russell et al. · 0 citations
Open access Aug 2026

Condensate Material Properties Influence Cargo Selection for Neuronal Extrusion via Large Extracellular Exopher Vesicles

Findings reveal that gel-like condensates with slower dynamics and higher viscoelasticity localize to exophers more avidly than dynamic liquid-like condensates, demonstrating a strong correlation between condensate dynamics and exopher recruitment.

Mayur Barai, E. Chuang, Shuo-Lin Weng et al. · 0 citations
Open access Jul 2026

Molecular Origins of pH Gradients in Charge-Regulated Biomolecular Condensates

Biomolecular condensates exhibit spontaneous electrochemical microenvironments characterized by asymmetric ion distributions and pH gradients that emerge from protein-sequence-dependent charge regulation. Despite their biological importance, mechanistic understanding of these microenvironments has been constrained by t...

Shuo-Lin Weng, Shiv Rekhi, Young C. Kim et al. · 0 citations

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