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Overall, it is found that spatial and other ecological factors can substantially affect the outcome of a confined CRISPR toxin-antidote drive release.
It is found that using more than one gRNA reduced the efficiency of inheritance, especially in females, and this results show that gene drive performance depends strongly on biological context and design choices, and that strategies must be carefully optimized in the target species.
2-locus TARE drives represent promising tools for effective and strongly confined population modification and are constructed with underdominance characteristics, yielding a higher introduction threshold even when drive performance is ideal.
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