James H. Hurley

DFCP1-containing ER microdomains mediate lysosomal membrane repair

Lysosomal membrane integrity is essential for preserving cellular homeostasis in response to different stressors. Upon lysosomal membrane permeabilization, cells activate several mechanisms for lysosomal membrane repair, including ESCRT proteins, phosphatidylinositol 4-phosphate (PI4P)-dependent lipid transfer from the...

A TBK1/ULK1 signalling axis couples lysosomal stress to TFEB activation.

Assembly and priming of the VPS4B AAA+ ATPase of the human ESCRT system

The cryo-electron microscopy structure of the full-length human VPS4B hexamer in six-membered helical “spiral staircase” states suggests a mechanism for substrate-dependent hexamerization and priming of VPS4 for its ESCRT-III remodeling activity.