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J. Ryder

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Open access Jul 2026

Cpf1(Cas12a)-based genome editing in the filamentous cyanobacterium Nostoc punctiforme

Cpf1-based genome editing tools were developed for N. punctiforme, and a single-step cloning strategy was devised, allowing for rapid assembly of editing plasmids, and improved conjugation protocols for genetic transfer from E. coli to N. punctiforme were implemented.

Jenna R. Ryder, Soohan Woo, Ailea A. Blahm et al. · 0 citations