Jérémie J. M. Marlhens

Releasing Protein Cargo from Synthetic Cells by Coupling Cell-Free Expression to Osmotic Actuation

Macromolecular release from synthetic cells often relies on membrane pores or channels. We explored whether internally generated osmotic stress could increase membrane permeability and promote release. Using double-emulsion microfluidics, we encapsulated an Escherichia coli cell-free expression system in Pluronic L121...