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Jerry Augustine

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Open access Aug 2026

The metal-uptake-deficient Escherichia coli strain GR536 contains the ϕ80 prophage

Growth conditions that limit the effects of ϕ80-dependent lysis when using GR536 to identify metal-uptake genes by complementation are clarified, specifically, removal of the antibiotic resistance markers and the avoidance of using intact pBAD30 as a negative control.

Ayuki Shimpo, Jerry Augustine, Luke Acton et al. · 0 citations