Jia Liu

[Efficient production of l- lysine by Escherichia coli through systems metabolic engineering].

Genome-scale overexpression screening identifies product tolerance and efflux transport as key determinants of high-level L-tryptophan production in Escherichia coli.

L-tryptophan is a high-value aromatic amino acid widely used in the food, feed, and pharmaceutical industries. However, large-scale microbial production is constrained by insufficient precursor supply and limited strain tolerance to high product concentrations. In this study, modular metabolic engineering was first emp...

Reprogramming energy system for growth and bioproduction from CO₂ and methanol in Escherichia coli

Microbial CO₂ assimilation provides a strategy for sustainable biomanufacturing. However, CO₂ reduction requires substantial energy input, while native energy systems are tightly regulated and extensively consumed by endogenous metabolism, limiting assimilation efficiency. Here, we design and construct an orthogonal en...

From laboratory to industry: Systematic approaches to the biomanufacturing of short-chain diols.

Short-chain diols (C2-C6) are key platform chemicals widely used in polyesters and polyurethanes; however, their large-scale adoption remains limited by cost, scalability, and process robustness. This review summarizes recent advances in microbial production from an integrated perspective encompassing chassis engineeri...

Metabolic flux reprogramming and protein engineering drive efficient l-arginine biosynthesis.

l-arginine is widely used in food, feed, pharmaceutical, and cosmetic industries. However, its industrial-scale biosynthesis is limited by insufficient coordination between metabolic regulation, pathway engineering, and fermentation optimization. In this study, an enzyme-constrained model (ec_iML1515) was used to ident...