A comprehensive and up-to-date overview of AI-driven PTM site prediction across more than ten PTM classes, covering single-PTM site prediction, multiple-PTM site prediction, inter-site crosstalk prediction, and functional prediction of modification sites is provided.
Jia-Yi Ran, Xiaohan Zhang, Yunze Wang et al.· Genomics, Proteomics & Bioin...· 0 citations
This protocol covers both single-gRNA (sgRNA) and dual-gRNA directed knockout systems and describes the procedure for constructing an Auxin-Inducible Degron (AID) knock-in-mediated system to induce the rapid degradation of a target protein in cells, thereby investigating its function.
Qian Tang, Jian Liu· Methods in molecular biology· 0 citations